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In order to determine if RMD controls F-actin organization within the pollen tube, F-actin arrays in wild type and rmd-1 mature pollen grains were observed using Alexa Fluor 488-phalloidin staining. The maximum length of the F-actin cables were shorter in the rmd-mutant pollen tubes compared to those in the wild type tubes. Strongly bundled actin filaments were present around the apertures of the wild type pollen grains although there was no accumulation of actin filaments around the apertures in the rmd-1 pollen grains. But, there was a lower density of F-actin observed in the tip region of the rmd-mutant tubes compared to the wild type tubes. RMD, which are type II formins, consist of a phosphatase, (PTEN)-like domain (responsible for protein localization), and FH1 and FH2 domains (promotes actin polymerization). A type of parsley not carried here previously -- perfect! The most striking difference is that stressed BIN can be used in past and past perfect sentences that do not correspond to uses of stressed BEEN in Standard American English.
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Today two types of Duffel bags are available, one is classic duffel, which opens & closes at the short end of the bag like laundry bags, on the other hand, modern duffel bags have a single zipper all the way across the bag's length. The maximum length of the single cables of F-actin filaments from the apical to the shank region of elongating pollen tubes were also measured to test the polarity within the pollen tube. By comparing the images of the control with pLat52::RMD-eGFP, it is observed that the single GFP was spread throughout the entire tube whereas RMD-eGFP accumulated in the tip region of the tube. The PTEN-eGFP signals were localized in the tip of the pollen tubes like the RMD-eGFP signals, whereas the FH1FH2-eGFP signals were present throughout the pollen tube and not localized in a polar manner. The heat from pyroclastic flow had simply boiled their brains until the pressure shattered their heads like eggshells. Heat also prevents inflammation, probably by increasing the secretion of cortisol and catecholamines, two hormones produced by the adrenal glands.
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